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Addgene inc yap, cox-2, lats1 shrna
Yap, Cox 2, Lats1 Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lats1+shrna/pegfp+c3+lats1/pm29037225-58-17-23
Average 90 stars, based on 1 article reviews

yap, cox-2, lats1 shrna - by Bioz Stars, 2026-09

90/100 stars

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Article Title: YAP transcriptionally regulates COX-2 expression and GCCSysm-4 (G-4), a dual YAP/COX-2 inhibitor, overcomes drug resistance in colorectal cancer.
Article Snippet: Antibodies to COX-2, YAP, LATS1, HRP-linked goat anti-mouse IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). .. DNA plasmids that encode wild type human YAP (hYAP, CMV2-YAP) and TEAD1 vector (pRK5-Myc-TEAD 1) and YAP, COX-2, LATS1 shRNA were obtained from Addgene (USA). ..

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Relationship between LATS1 protein expression (detected by IHC) and clinic-pathological factors in 102 CRC patients

Journal: Journal of Cancer

Article Title: LATS1 exerts tumor suppressor functions via targeting Gli1 in colorectal cancer

doi: 10.7150/jca.62211

Figure Lengend Snippet: Relationship between LATS1 protein expression (detected by IHC) and clinic-pathological factors in 102 CRC patients

Article Snippet: The Lentivirus vectors carrying LATS1 overexpression or short hairpin RNA (shRNA) targeting LATS1 and their control vectors were invented by Genechem Company (Shanghai, China).

Techniques: Expressing

LATS1 expression in human CRC tissues. (A) Data from the GEPIA database showed that LATS1 mRNA was downregulated in CRC tumor tissues (T) compared to normal controls (N), COAD colon adenocarcinoma, READ rectal adenocarcinoma. (B) IHC staining for LATS1 in CRC tissues and peritumoural normal tissues. (C) The IHC score of LATS1 in CRC tissue samples (T) and normal tissues (N). (D-I) LATS1 expression in CRC tumor tissues of different tumor size ( D ), depth of invasion ( E ), lymph node metastasis ( F ), TNM stage ( G ), tumor location ( H ) and degree of differentiation (I). NS, nonsignificant, * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Journal of Cancer

Article Title: LATS1 exerts tumor suppressor functions via targeting Gli1 in colorectal cancer

doi: 10.7150/jca.62211

Figure Lengend Snippet: LATS1 expression in human CRC tissues. (A) Data from the GEPIA database showed that LATS1 mRNA was downregulated in CRC tumor tissues (T) compared to normal controls (N), COAD colon adenocarcinoma, READ rectal adenocarcinoma. (B) IHC staining for LATS1 in CRC tissues and peritumoural normal tissues. (C) The IHC score of LATS1 in CRC tissue samples (T) and normal tissues (N). (D-I) LATS1 expression in CRC tumor tissues of different tumor size ( D ), depth of invasion ( E ), lymph node metastasis ( F ), TNM stage ( G ), tumor location ( H ) and degree of differentiation (I). NS, nonsignificant, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The Lentivirus vectors carrying LATS1 overexpression or short hairpin RNA (shRNA) targeting LATS1 and their control vectors were invented by Genechem Company (Shanghai, China).

Techniques: Expressing, Immunohistochemistry

LATS1 expression in human CRC cell lines. (A-B) Western blot (A) and qRT-PCR (B) analysis of LATS1 expression in CRC cell lines (HCT8, HCT116, LoVo, RKO, SW480, SW620, CaCo2 and DLD-1). (C-D) Western blot (C) and qRT-PCR (D) analysis of LATS1 expression in HCT116 and SW480 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (E-F) Western blot (E) and qRT-PCR (F) analysis of LATS1 expression in SW620 and CaCo2 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). *** P < 0.001.

Journal: Journal of Cancer

Article Title: LATS1 exerts tumor suppressor functions via targeting Gli1 in colorectal cancer

doi: 10.7150/jca.62211

Figure Lengend Snippet: LATS1 expression in human CRC cell lines. (A-B) Western blot (A) and qRT-PCR (B) analysis of LATS1 expression in CRC cell lines (HCT8, HCT116, LoVo, RKO, SW480, SW620, CaCo2 and DLD-1). (C-D) Western blot (C) and qRT-PCR (D) analysis of LATS1 expression in HCT116 and SW480 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (E-F) Western blot (E) and qRT-PCR (F) analysis of LATS1 expression in SW620 and CaCo2 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). *** P < 0.001.

Article Snippet: The Lentivirus vectors carrying LATS1 overexpression or short hairpin RNA (shRNA) targeting LATS1 and their control vectors were invented by Genechem Company (Shanghai, China).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Stable Transfection, Transfection, Control, shRNA, Plasmid Preparation

LATS1 plays tumor-suppresive roles in CRC. (A) Colony formation assay in HCT116 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (B) Colony formation assay in SW620 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). (C) Transwell assay in HCT116 cells (NC vs. KD). (D) Transwell assay in SW620 cells (VEC vs. OE). ** P < 0.01.

Journal: Journal of Cancer

Article Title: LATS1 exerts tumor suppressor functions via targeting Gli1 in colorectal cancer

doi: 10.7150/jca.62211

Figure Lengend Snippet: LATS1 plays tumor-suppresive roles in CRC. (A) Colony formation assay in HCT116 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (B) Colony formation assay in SW620 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). (C) Transwell assay in HCT116 cells (NC vs. KD). (D) Transwell assay in SW620 cells (VEC vs. OE). ** P < 0.01.

Article Snippet: The Lentivirus vectors carrying LATS1 overexpression or short hairpin RNA (shRNA) targeting LATS1 and their control vectors were invented by Genechem Company (Shanghai, China).

Techniques: Colony Assay, Stable Transfection, Transfection, Control, shRNA, Plasmid Preparation, Transwell Assay

LATS1 inhibits CRC cell proliferation and migration in a Gli1-dependent manner. (A) Western blot analysis of Gli1 expression in HCT116 and SW480 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (B) Western blot analysis of Gli1 expression in SW620 and CaCo2 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). (C) Western blot analysis of Gli1 expression in LATS1-depleted HCT116 cells with or without Gli1 siRNA treatment. (D) Colony formation assay in LATS1-depleted HCT116 cells with or without Gli1 siRNA treatment. (E) Transwell assay in LATS1-depleted HCT116 cells with or without Gli1 siRNA treatment. NS, nonsignificant, ** P < 0.01, *** P < 0.001.

Journal: Journal of Cancer

Article Title: LATS1 exerts tumor suppressor functions via targeting Gli1 in colorectal cancer

doi: 10.7150/jca.62211

Figure Lengend Snippet: LATS1 inhibits CRC cell proliferation and migration in a Gli1-dependent manner. (A) Western blot analysis of Gli1 expression in HCT116 and SW480 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (B) Western blot analysis of Gli1 expression in SW620 and CaCo2 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). (C) Western blot analysis of Gli1 expression in LATS1-depleted HCT116 cells with or without Gli1 siRNA treatment. (D) Colony formation assay in LATS1-depleted HCT116 cells with or without Gli1 siRNA treatment. (E) Transwell assay in LATS1-depleted HCT116 cells with or without Gli1 siRNA treatment. NS, nonsignificant, ** P < 0.01, *** P < 0.001.

Article Snippet: The Lentivirus vectors carrying LATS1 overexpression or short hairpin RNA (shRNA) targeting LATS1 and their control vectors were invented by Genechem Company (Shanghai, China).

Techniques: Migration, Western Blot, Expressing, Stable Transfection, Transfection, Control, shRNA, Plasmid Preparation, Colony Assay, Transwell Assay

LATS1 regulates YAP1 expression and mTOR signaling pathway. (A) Western blot analysis of the indicated proteins in HCT116 and SW480 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (B) Western blot analysis of the indicated proteins in SW620 and CaCo2 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). * P < 0.05, ** P < 0.01.

Journal: Journal of Cancer

Article Title: LATS1 exerts tumor suppressor functions via targeting Gli1 in colorectal cancer

doi: 10.7150/jca.62211

Figure Lengend Snippet: LATS1 regulates YAP1 expression and mTOR signaling pathway. (A) Western blot analysis of the indicated proteins in HCT116 and SW480 cells stably transfected with control-shRNA (NC) or shRNA against LATS1 (KD). (B) Western blot analysis of the indicated proteins in SW620 and CaCo2 cells stably transfected with empty vector (VEC) or plasmids encoding human LATS1 (OE). * P < 0.05, ** P < 0.01.

Article Snippet: The Lentivirus vectors carrying LATS1 overexpression or short hairpin RNA (shRNA) targeting LATS1 and their control vectors were invented by Genechem Company (Shanghai, China).

Techniques: Expressing, Western Blot, Stable Transfection, Transfection, Control, shRNA, Plasmid Preparation

LATS1 inhibition with RNA interference suppresses the promoter activities of anterior pituitary hormones. (A) GH3 cells were transfected with a scrambled siRNA (control) or four different siRNA against rat Lats1 for 48 h. Immunoblot shows the knockdown efficacy and the impact of decreased LATS1 protein on YAP phosphorylation at Ser127 and total YAP and TAZ protein levels. (B) Effect of Lats1 knockdown on basal Prl and Gh promoter activity. Data are luciferase to β-galactosidase ratio, means ± standard deviation of three experiments (each in triplicate) presented as percentage of scrambled control. RLA, relative luciferase activity. * P < 0.05 to scrambled vehicle control. (C) Effect of Lats1 knockdown on forskolin-induced (10 µM, 6 h treatment) Gh promoter activity. Data are means ± standard deviation of three triplicates and presented as percentage of scrambled vehicle control. * P < 0.05 and *** P < 0.001 vs scrambled vehicle control, # P < 0.05 vs siLats1 vehicle control. Veh, vehicle – the carrier in which forskolin was diluted. (D) Lats1 knockdown does not affect cell proliferation (WST-1 colorimetric assay). Data are absorbance at OD450nm presented as percentage of scrambled siRNA control.

Journal: Endocrine-Related Cancer

Article Title: Non-secreting pituitary tumours characterised by enhanced expression of YAP/TAZ

doi: 10.1530/ERC-18-0330

Figure Lengend Snippet: LATS1 inhibition with RNA interference suppresses the promoter activities of anterior pituitary hormones. (A) GH3 cells were transfected with a scrambled siRNA (control) or four different siRNA against rat Lats1 for 48 h. Immunoblot shows the knockdown efficacy and the impact of decreased LATS1 protein on YAP phosphorylation at Ser127 and total YAP and TAZ protein levels. (B) Effect of Lats1 knockdown on basal Prl and Gh promoter activity. Data are luciferase to β-galactosidase ratio, means ± standard deviation of three experiments (each in triplicate) presented as percentage of scrambled control. RLA, relative luciferase activity. * P < 0.05 to scrambled vehicle control. (C) Effect of Lats1 knockdown on forskolin-induced (10 µM, 6 h treatment) Gh promoter activity. Data are means ± standard deviation of three triplicates and presented as percentage of scrambled vehicle control. * P < 0.05 and *** P < 0.001 vs scrambled vehicle control, # P < 0.05 vs siLats1 vehicle control. Veh, vehicle – the carrier in which forskolin was diluted. (D) Lats1 knockdown does not affect cell proliferation (WST-1 colorimetric assay). Data are absorbance at OD450nm presented as percentage of scrambled siRNA control.

Article Snippet: Cells were transfected with SuperFect (Qiagen) following the manufacturer’s instructions. siRNA were against rat Lats1 (OriGene and Santa Cruz Biotechnology); a mix of scrambled non-specific siRNA was used as control.

Techniques: Inhibition, Transfection, Control, Western Blot, Knockdown, Phospho-proteomics, Activity Assay, Luciferase, Standard Deviation, Colorimetric Assay

( A ) Differential protein expression levels (low or high) of LATS1 were examined in GC tissues and ANTT by IHC staining (magnification, 200×). ( B ) Western blotting analysis of LATS1 expression in GC cell lines. ( C ) Low expression of LATS1 was markedly correlated with the overall survival and recurrence free survival of the GC patients. These data were analyzed using Kaplan-Meier survival analysis between the GC patients with low LATS1 expression and high LATS1 expression according to the immunostaining scores.

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: ( A ) Differential protein expression levels (low or high) of LATS1 were examined in GC tissues and ANTT by IHC staining (magnification, 200×). ( B ) Western blotting analysis of LATS1 expression in GC cell lines. ( C ) Low expression of LATS1 was markedly correlated with the overall survival and recurrence free survival of the GC patients. These data were analyzed using Kaplan-Meier survival analysis between the GC patients with low LATS1 expression and high LATS1 expression according to the immunostaining scores.

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Expressing, Immunohistochemistry, Western Blot, Immunostaining

The expression of LATS1 in human GC tissues

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: The expression of LATS1 in human GC tissues

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Expressing

The correlation of LATS1 protein expression with clinicopathologic features in GC patients

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: The correlation of LATS1 protein expression with clinicopathologic features in GC patients

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Expressing

Summary of univariate and multivariate Cox regression analysis of overall survival duration

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: Summary of univariate and multivariate Cox regression analysis of overall survival duration

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Expressing

Summary of univariate and multivariate Cox regression analysis of recurrence rate duration

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: Summary of univariate and multivariate Cox regression analysis of recurrence rate duration

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Expressing

( A ) LATS1 mRNA and ( B ) protein expression were respectively detected by Real-time PCR and Western blotting in SGC-7901 and HGC-27 cells transfected with LATS1. ( C ) The effect of LATS1 overexpression on cell proliferative activity was estimated by MTT assay, indicating that overexpression of LATS1 reduced cell growth after LATS1 transfection for 72 h. ( D ) Representative micrographs of Transwell invasion assay were indicated in SGC-7901 and HGC-27 cells transfected with LATS1 (magnification, 200×). ( E ) Overexpression of LATS1 decreased cell invasive potential compared with the NC group. ( F ) Flow cytometry analysis of early apoptotic rate in SGC-7901 and HGC-27 cells after LAST1 transfection for 48 h. ( G ) Overexpression of LATS1 induced cell apoptosis compared with the NC group. ( H ) Flow cytometry analysis of cell cycle distribution in SGC-7901 and HGC-27 cells transfected with LATS1. ( I ) Overexpression of LATS1 induced cell cycle arrest at S phase compared with the NC group (** P < 0.01). The data were shown as the means ± SD of three independent experiments. ** P < 0.01.

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: ( A ) LATS1 mRNA and ( B ) protein expression were respectively detected by Real-time PCR and Western blotting in SGC-7901 and HGC-27 cells transfected with LATS1. ( C ) The effect of LATS1 overexpression on cell proliferative activity was estimated by MTT assay, indicating that overexpression of LATS1 reduced cell growth after LATS1 transfection for 72 h. ( D ) Representative micrographs of Transwell invasion assay were indicated in SGC-7901 and HGC-27 cells transfected with LATS1 (magnification, 200×). ( E ) Overexpression of LATS1 decreased cell invasive potential compared with the NC group. ( F ) Flow cytometry analysis of early apoptotic rate in SGC-7901 and HGC-27 cells after LAST1 transfection for 48 h. ( G ) Overexpression of LATS1 induced cell apoptosis compared with the NC group. ( H ) Flow cytometry analysis of cell cycle distribution in SGC-7901 and HGC-27 cells transfected with LATS1. ( I ) Overexpression of LATS1 induced cell cycle arrest at S phase compared with the NC group (** P < 0.01). The data were shown as the means ± SD of three independent experiments. ** P < 0.01.

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Over Expression, Activity Assay, MTT Assay, Transwell Invasion Assay, Flow Cytometry

( A ) LATS1 mRNA and ( B ) protein expression were respectively examined by Real-time PCR and Western blotting in BGC-823 and AGS cells transfected with shLATS1. ( C ) The effect of LATS1 knockdown on cell proliferative activity was evaluated by MTT assay, showing that LATS1 knockdown prompted cell growth after shLATS1 transfection for 72 h. ( D ) Representative micrographs of Transwell invasion assay were indicated in BGC-823 and AGS cells transfected with shLATS1 (magnification, 200×). ( E ) Knockdown of LATS1 enhanced cell invasive potential compared with the NC group. ( F ) Flow cytometry analysis of early apoptotic rate in BGC-823 and AGS cells after shLAST1 treatment for 48 h. ( G ) Knockdown of LATS1 cut down cell apoptosis compared with the NC group. ( H ) Flow cytometry analysis of cell cycle distribution in BGC-823 and AGS cells treated with shLATS1. ( I ) Knockdown of LATS1 decreased the cell cycle arrest at S phase compared with the NC group. The data were shown as the means ± SD of three independent experiments. * P < 0.05, ** P < 0.01.

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: ( A ) LATS1 mRNA and ( B ) protein expression were respectively examined by Real-time PCR and Western blotting in BGC-823 and AGS cells transfected with shLATS1. ( C ) The effect of LATS1 knockdown on cell proliferative activity was evaluated by MTT assay, showing that LATS1 knockdown prompted cell growth after shLATS1 transfection for 72 h. ( D ) Representative micrographs of Transwell invasion assay were indicated in BGC-823 and AGS cells transfected with shLATS1 (magnification, 200×). ( E ) Knockdown of LATS1 enhanced cell invasive potential compared with the NC group. ( F ) Flow cytometry analysis of early apoptotic rate in BGC-823 and AGS cells after shLAST1 treatment for 48 h. ( G ) Knockdown of LATS1 cut down cell apoptosis compared with the NC group. ( H ) Flow cytometry analysis of cell cycle distribution in BGC-823 and AGS cells treated with shLATS1. ( I ) Knockdown of LATS1 decreased the cell cycle arrest at S phase compared with the NC group. The data were shown as the means ± SD of three independent experiments. * P < 0.05, ** P < 0.01.

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Knockdown, Activity Assay, MTT Assay, Transwell Invasion Assay, Flow Cytometry

( A ) YAP-overexpressed vector was transfected into LATS1-pretreated GC cell lines (SGC-7901 and HGC-27). After transfection for 72 h, cell proliferative activity was assessed by MTT assay, indicating that YAP overexpression weakened the therapeutic effects of LATS1 on cell growth. ( B , C ) After YAP-overexpressed vector was transfected into LATS1-treated GC cell lines (SGC-7901 and HGC-27), cell invasive potential was determined by Transwell assay, demonstrating that YAP lowered the anti-invasion effect of LATS1 in GC cells. ( D ) Western blotting analysis of the YAP, p-YAP, CTGF, MMP-9, Bax and CyclinD1 expression in YAP-transfected SGC-7901 and HGC-27 cells treated with LATS1. ( E ) Immunofluorescence analysis of the location of YAP in SGC-7901 and HGC-27 cells transfected with LATS1. The data were shown as the means ± SD of three independent experiments. ** P < 0.01.

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: ( A ) YAP-overexpressed vector was transfected into LATS1-pretreated GC cell lines (SGC-7901 and HGC-27). After transfection for 72 h, cell proliferative activity was assessed by MTT assay, indicating that YAP overexpression weakened the therapeutic effects of LATS1 on cell growth. ( B , C ) After YAP-overexpressed vector was transfected into LATS1-treated GC cell lines (SGC-7901 and HGC-27), cell invasive potential was determined by Transwell assay, demonstrating that YAP lowered the anti-invasion effect of LATS1 in GC cells. ( D ) Western blotting analysis of the YAP, p-YAP, CTGF, MMP-9, Bax and CyclinD1 expression in YAP-transfected SGC-7901 and HGC-27 cells treated with LATS1. ( E ) Immunofluorescence analysis of the location of YAP in SGC-7901 and HGC-27 cells transfected with LATS1. The data were shown as the means ± SD of three independent experiments. ** P < 0.01.

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Plasmid Preparation, Transfection, Activity Assay, MTT Assay, Over Expression, Transwell Assay, Western Blot, Expressing, Immunofluorescence

( A ) Representative photographs of GC and metastatic liver tumors and HE staining of liver tissue derived from SGC-7901-LATS1 or SGC-7901-NC cells in SCID mice. ( B ) At the end of the experiment, the average volume and weight of gastric tumors in LATS1 group were significantly lower than those of the NC group. The number of metastatic liver tumor nodules and the average liver weight in LATS1 group were much lower than those of the NC group). ( C ) IHC analysis of the protein expression of YAP, CTGF, MMP-9, Bax and CyclinD1 in gastric tumors and metastatic liver tumors. The data were shown as the means ± SD of three independent experiments. ** P < 0.01.

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: ( A ) Representative photographs of GC and metastatic liver tumors and HE staining of liver tissue derived from SGC-7901-LATS1 or SGC-7901-NC cells in SCID mice. ( B ) At the end of the experiment, the average volume and weight of gastric tumors in LATS1 group were significantly lower than those of the NC group. The number of metastatic liver tumor nodules and the average liver weight in LATS1 group were much lower than those of the NC group). ( C ) IHC analysis of the protein expression of YAP, CTGF, MMP-9, Bax and CyclinD1 in gastric tumors and metastatic liver tumors. The data were shown as the means ± SD of three independent experiments. ** P < 0.01.

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Staining, Derivative Assay, Expressing

YAP is required for LATS1-induced inhibition of GC cell proliferation and invasion through upregulation of CTGF, MMP-9 and CyclinD1 expression and downregulation of Bax expression.

Journal: Oncotarget

Article Title: Loss of large tumor suppressor 1 promotes growth and metastasis of gastric cancer cells through upregulation of the YAP signaling

doi: 10.18632/oncotarget.7568

Figure Lengend Snippet: YAP is required for LATS1-induced inhibition of GC cell proliferation and invasion through upregulation of CTGF, MMP-9 and CyclinD1 expression and downregulation of Bax expression.

Article Snippet: To silence the expression of LATS1, short hairpin RNA (shRNA) sequence targeting LATS1 gene (clone: ATCCTCGACGAGAGCAGA) were purchased from Genechem (Shanghai, PR, China).

Techniques: Inhibition, Expressing

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yap, cox-2, lats1 shrna (Addgene inc)

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